Stella Alimperti, Ph.D.
Styliani (Stella) Alimperti, Ph.D.
- 3D printing
- Vascular tissue engineering
- Rare diseases
- Personalized medicine
- Synthetic Biology
Styliani Alimperti, Ph.D. Curriculum Vitae (PDF) (coming soon)
Dr. Alimperti’s scientific interests are focused on developing novel in situ and ex vivo systems with potential translation into new therapies for dental diseases. Her ongoing research entails state-of-the-art engineering methods: organ-on-a-chip, 3D printing, stem cell engineering andsynthetic biology tools. These methods can also find utility in personalized medicine. Specifically, organ-on-a-chip technology is employed to build multicellular level functional units of an organ through microengineering. A functional biomimetic microfluidic unit with the complex architecture and tunable biophysical and biochemical properties is being developed. This in vitro platform is ideal to mimic the gum pathophysiology (gum-on-a-chip) and to identify novel drugs targeting gum disease. In addition, engineering cell therapy technologies (iPS-driven cells/adult cells) in combination with the molecular biology strategies (CrispR) are being used to investigate development of gum diseases. Ultimately, differentiated functional cells will be utilized for personalized medical treatment of gum diseases. Integration of organ-on-a-chip and organ cell specific cell technologies with 3D printing technologies will enable the 3D-printing autologous organ for transplantation.
Selected Publications (See NIH Public Access Listing)
- Alimperti S, Mirabella T, Bajaj V, Polacheck W, Pirone DM, Duffield J, et al. Three-dimensional biomimetic vascular model reveals a RhoA, Rac1, and N-cadherin balance in mural cell-endothelial cell-regulated barrier function. Proc Natl Acad Sci U S A. 2017;114(33):8758-63. Epub 2017/08/03. doi: 10.1073/pnas.1618333114. PubMed PMID: 28765370; PubMed Central PMCID: PMCPMC5565405.
- Dang LTH, Aburatani T, Marsh GA, Johnson BG, Alimperti S, Yoon CJ, et al. Hyperactive FOXO1 results in lack of tip stalk identity and deficient microvascular regeneration during kidney injury. Biomaterials. 2017;141:314-29. Epub 2017/07/18. doi: 10.1016/j.biomaterials.2017.07.010. PubMed PMID: 28711779; PubMed Central PMCID: PMCPMC5567800.
- McCurley A, Alimperti S, Campos-Bilderback SB, Sandoval RM, Calvino JE, Reynolds TL, et al. Inhibition of alphavbeta5 Integrin Attenuates Vascular Permeability and Protects against Renal Ischemia-Reperfusion Injury. J Am Soc Nephrol. 2017;28(6):1741-52. Epub 2017/01/08. doi: 10.1681/ASN.2016020200. PubMed PMID: 28062569; PubMed Central PMCID: PMCPMC5461783.
- Row S, Liu Y, Alimperti S, Agarwal SK, Andreadis ST. Cadherin-11 is a novel regulator of extracellular matrix synthesis and tissue mechanics. J Cell Sci. 2016;129(15):2950-61. Epub 2016/06/18. doi: 10.1242/jcs.183772. PubMed PMID: 27311482; PubMed Central PMCID: PMCPMC5004872.
- Alimperti S, Andreadis ST. CDH2 and CDH11 act as regulators of stem cell fate decisions. Stem Cell Res. 2015;14(3):270-82. Epub 2015/03/17. doi: 10.1016/j.scr.2015.02.002. PubMed PMID: 25771201; PubMed Central PMCID: PMCPMC4439315.
- Alimperti S, You H, George T, Agarwal SK, Andreadis ST. Cadherin-11 regulates both mesenchymal stem cell differentiation into smooth muscle cells and the development of contractile function in vivo. J Cell Sci. 2014;127(Pt 12):2627-38. Epub 2014/04/18. doi: 10.1242/jcs.134833. PubMed PMID: 24741067; PubMed Central PMCID: PMCPMC4058109.
- Alimperti S, Lei P, Wen Y, Tian J, Campbell AM, Andreadis ST. Serum-free spheroid suspension culture maintains mesenchymal stem cell proliferation and differentiation potential. Biotechnol Prog. 2014;30(4):974-83. Epub 2014/03/13. doi: 10.1002/btpr.1904. PubMed PMID: 24616445; PubMed Central PMCID: PMCPMC5081183.
- Alimperti S, Lei P, Tian J, Andreadis ST. A novel lentivirus for quantitative assessment of gene knockdown in stem cell differentiation. Gene Ther. 2012;19(12):1123-32. Epub 2012/01/14. doi: 10.1038/gt.2011.208. PubMed PMID: 22241174.
- Tian J, Alimperti S, Lei P, Andreadis ST. Lentiviral microarrays for real-time monitoring of gene expression dynamics. Lab Chip. 2010;10(15):1967-75. Epub 2010/06/04. doi: 10.1039/c003153d. PubMed PMID: 20520864.
- Nguyen, D.H., Lee, E., Alimperti, S., Wong, A., Stanger, B., Chen, C.S. "Pancreatic ductal adenocarcinoma replaces endothelium during tissue invasion" (under review in PNAS).
- Anastasiadou, E., Stroopinsky, D., Alimperti, S., et.al “Epstein-Barr virus encoded EBNA2 alters immune checkpoint PD-L1 expression by downregulating miR-34a in B cell lymphomas” (under review in Blood)
- Lee, E., Polachek, W., Alimperti, S., Nguyen, D.T, Chen, C.S. “A Biomimetic on-Chip Model to Reconstitute Lymphedema” (manuscript in preparation).