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Dr. Gili Kaufman
Gili Kaufman, Ph.D.

Gili Kaufman, Ph.D.

Research Interests

  • Oral and Dental Soft Tissue Bioregeneration
  • Scaffold-Free 3D Cell and Tissue Models
  • Molecular and Cellular Biomarkers
  • Pathogenicity of Oral Microbes
  • Cytotoxic Mechanisms of Polymeric Dental Materials

Gili Kaufman, Ph.D. Curriculum Vitae (PDF) 


Cytotoxicity
Testing the cytotoxicity of leachable polymers on the development of 3D gingival fibroblasts.
VRC Kaufman Host Pathogen Interactions
Host-Pathogen Interactions
3D dental pulp cells challenged with endodontic pathogen (Red: cellular F-actin, Green: bacterial biofilms).
VRC Kaufman Tissue Regeneration
Tissue Regeneration
Directing the rearrangement of gingival fibroblast layers by using natural protein coated nano-fibers (blue: fibers, red: cellular F-actin).

Currently, some of the projects in Dr. Kaufman’s lab involve the development of Organotypic (3D) cell tissue cultures/microtissues developed from soft oral and dental derived stem cells  as an (i) advanced cell products utilized as building blocks in tissue regeneration and bioprinting applications and (ii) unique 3D biomimetic cell models to investigate the effect of oral pathogens and toxic materials leaching from dental composites on the structural, regeneration and differentiation capacities of oral and dental soft tissues. The following last two models are also used to screen for cellular genetic and epigenetic markers and study their regulatory mechanisms. Other collaborative projects investigate: (i) The resistance of innovative dental composites to microbial hydrolytic enzyme degradations and compatibility to the surrounding cell populations. (ii) The effect of air-brushing technique and collagen I and fibrin coated PLGA nanofibers on the regeneration of gingival connective tissue in open wounds or injured tissue. (iii) Development of mucosa-like micro tissue in a biomimetic microfluidic device for determining the transfer and absorbance of leaching dental composite monomers.

Selected Publications (See NIH Public Access Listing)

  • G. Kaufman, P. Liu, J.L. Leibowitz: Identification of novel functional regions within the spike glycoprotein of MHV-A59 based on a bioinformatics approach. Virus Res. 2014, 189, 177-188.
  • Gonzalez-Bonet, G. Kaufman, Y. Yang, C. Wong, A. Jackson, G. Huyang, R.L. Bowen, J. Sun:  Preparation of Dental Resins Resistant to Enzymatic and Hydrolytic Degradation in Oral Environments.  Biomacromolecules. 2015 Oct 12;16(10):3381-8.
    Supplemental coverage of work: Chemical & Engineering News, October 12, 2015. (http://cen.acs.org/articles/93/web/2015/09/New-Dental-Material-Resists-Degradation.html).
  • W. Tutak, G. Kaufman, G. Gelven, C. Markle, C. Maczka: Uniform, fast, high concentration delivery of bone marrow stromal cells and gingival fibroblasts by gas-brushing. Biomedical Physics & Engineering Express. April 2016  Volume 2; doi:10.1088/2057-1976/2/3/035007.
  • G. Kaufman, L. Nunes, A. Eftimiades, W. Tutak:  Enhancing 3D structure of adherent gingival fibroblasts and spheroids via fibrous protein-based hydrogel cover. Cells, Tissues, Organs, in press.
  • G. Kaufman and D. Skrtic: Structural and recovery mechanisms of 3D dental pulp cell cultures challenged with Streptococcus mutans in extracellular matrix environment. J. Med. Microbiol. 2016, submitted.